Table 2. Solvent Gradient Conditions for UPLC-PDA Analysis

Time(minutes)	0.1% FA/water(%)	0.1% FA/acetonitrile(%)	Flow rate(㎖/min)
0	98	2	0.40
1.0	98	2	0.40
2.0	95	5	0.40
3.0	85	15	0.40
5.0	75	25	0.40
6.0	60	40	0.40
8.0	50	50	0.40
9.0	20	80	0.40
10.0	10	90	0.40
12.0	2	98	0.40
14.0	98	2	0.40
16.0	98	2	0.40

UPLC: Ultra Performance Liquid Chromatography