Table 2. Solvent Grade Conditions for UPLC-PDA Analysis

Time(min)	0	1.0	2.0	4.0	6.0	9.0	13.0	14.0	15.0	16.0
0.1% FA/water(%)	98	98	90	70	50	30	10	1	98	98
0.1% FA/acetonitrile(%)	2	2	10	30	50	70	90	99	2	2
Flow rate(㎖/min)	0.40	0.40	0.40	0.40	0.40	0.40	0.40	0.40	0.40	0.40