Table 1 Primers Sequence
Gene
Direction
Sequence (5' → 3')
Size (bp)
HAS2
Forward
GCT ACC AGT TTA TCC AAA CG (20 mer)
393
Reverse
GTG ACT CAT CTG TCT CAC CG (20 mer)
GAPDH
Forward
ATT GTT GCC ATC AAT GAC CC (20 mer)
546
Reverse
AGT AGA GGC AGG GAT GAT GT (20 mer)
PCR 조건은 다음과 같다. 94℃ 15 min, 32~35 cycles: 94℃ 30s 50℃ 30s 72℃ 60s 72℃ 10 min 최종 합성.